HPLC Analysis of Ergosterol in Vacuum Cleaner Dust on Chromolith(R) Performance 100-2mm

HPLC Analysis of Ergosterol in Vacuum Cleaner Dust on Chromolith® Performance 100-2mm

Conditions

sample preparation See "Analysis Note."
column Chromolith® Performance, 100 x 2 mm (1.52006.0001)
column temp. 23 °C
mobile phase [A] methanol; [B] Water; (98:2, A:B)
flow rate 190 μL/min
pressure 595 psi 41 Bar
sample Standard: Ergosterol; Matrix: Dust of a vacuum cleaner
injection 1 μL
detector 282 nm cell: 1.4 μL

Description

Analysis Note Sample preparation: The dust of a vacuum cleaner was sieved through a normal kitchen sieve. 500 mg of the sieved dust was suspended with 5.4 mL potassium hydroxide (10%) in methanol and stirred over 2h by 80°C.After cooling down, the suspension  was centrifuged. 4mL of the obtained solution was filtered through a 0.45 μm  membrane filter and washed with 2mL water. The combined solutions were then extracted two times with 2 mL n-hexane. The n-hexane was evaporated with nitrogen gas and the residue was resolved in 1 mL methanol. The  final solution  were  spiked with 100 μL of Ergosterol  (1 mg/mL) standard solution .
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suitability application for HPLC

Materials

     
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