HPLC Analysis of Ethacrynic Acid on Chromolith(R) HighResolution RP-18endcapped, 100 x 4.6 mm

HPLC Analysis of Ethacrynic Acid on Chromolith® HighResolution RP-18endcapped, 100 x 4.6 mm

Conditions

column Chromolith® HighResolution-18 endcapped, 100 x 4.6mm (1.52022.0001)
column temp. 45 °C
mobile phase [A] 10 g of acetic acid and dilute to 1L with water. Adjust pH to 4.5 with dilute ammonia ; [B] methanol;
gradient 50% A held for 4 min; to 20% A in 10 min; held at 20% A for 2 min; to 50% A in 0.8 min; held at 50% A for 3.2 min (Original: Time (Min) % A % B; 0.0, 50, 50; 4.0, 50, 50; 14.0, 20, 80; 16.0, 20, 80; 16.8, 50, 50; 20.0, 50, 50; )
flow rate 1 mL/min
pressure 1029-826 psi 71--> 57 Bar
sample Weigh 100 mg of ethacrynic acid in 100 ml volumetric flask. Add each impurity standard to get a 1 ppm level. Dilute with diluent.
injection 10 μL
detector 280 nm

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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