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HPLC Analysis of Finasteride Tablets on Chromolith(R) HighResolution RP-18 endcapped 100 x 4.6mm

HPLC Analysis of Finasteride Tablets on Chromolith® HighResolution RP-18 endcapped 100 x 4.6mm


sample preparation Dissolve an accurately weighed quantity of USP Finasteride RS in Diluting solution, and dilute quantitatively, and stepwise if necessary, with Diluting solution to obtain a solution having a known concentration of about 100 μg per mL.
column Chromolith HighResolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 45 °C
mobile phase [A] acetonitrle; [B] 2.5 mM phosphoric acid in Water; (40:60,:A:B)
flow rate 1.5 mL/min
pressure 624 psi 43 Bar
sample Finasteride RS 100 μg/mL (100 ppm) (Diluent: Acetonitrile and water 70:30 (v/v))
injection 20 μL
detector 240 nm cell: 11 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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