HPLC Analysis of Folic Acid Syrup on Chromolith High Resolution RP-18

HPLC Analysis of Folic Acid Syrup on Chromolith High Resolution RP-18

Conditions

sample preparation Pipette out 15 ml of syrup in 50 ml volumetric flask. Add diluent to make up the volume.Sonicate for 30 min. Fiter through 0.2 μm filter paper.
column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 1.5 g of hexane 1-sulfonate in 1000 ml water containing 10 ml of glacial acetic acid; [B] acetonitrile;
flow rate 1.0 mL/min
pressure 1203 psi 83 Bar
sample 10 ppm in diluent (Diluent: Dissolve 6.8 g of KH2PO4 in 1000 ml water. Adjust pH to 6.8 with 10% NaOH solution)
injection 10 μL
detector 277 nm cell: 10 μL

Description

Featured Industry Life Science and Biopharma
suitability application for HPLC

Materials

     
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