HPLC Analysis of Ganciclovir Injection Solution on Chromolith(R) HighResolution RP-18 endcapped 100 x 4.6mm

HPLC Analysis of Ganciclovir Injection Solution on Chromolith® HighResolution RP-18 endcapped 100 x 4.6mm

Conditions

sample preparation Transfer 20.0 mL of standard stock solution (250 μg/mL of USP Ganciclovir RS in water) and 10.0 mL of the Internal standard solution to a 100 mL volumetric flask. Dilute with mobile phase to volume.
column Chromolith HighResolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase 1.4 g/L of monobasic ammonium phosphate and 2.0 g/L of o-phosphoric acid (85%) in water
flow rate 2.0 mL/min
pressure 1407 psi (97 Bar)
sample Ganciclovir RS, 0.05 mg/mL of USP Ganciclovir RS (Diluent: water)
injection 10 μL
detector 254 nm cell: 11 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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