HPLC Analysis of Gingko Leaves Tablets on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Gingko Leaves Tablets on Purospher® STAR RP-18 endcapped


sample preparation Take 10 drug tablets, grind. Take propraite amount of drug powder (Contained 9.6 mg flavonol glycoside ),add into 25mL methanol-25% HCL aqueous solution (4:1, v/v).Shake well, put into water bath to heat and reflux for 30 min.  Cool rapidly to room temperature, transfer to 50-mL volumetric flask1,add methanol to volume. Shake well filtrate, inject the filtrate for HPLC analysis.
column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
column temp. 30 °C
mobile phase [A] 0.4% H3PO4 aqueous solution; [B] methanol; (50:50, A:B)
flow rate 1 mL/min
pressure 2755 psi 190 Bar
sample Dissolve appropriate amounte of Kaempferide, Isophamnetin and Quercetin in methanol to obtain a 30 μg/mL (30 ppm) of each in final solution
injection 10 μL
detector 360 nm cell: 1μL/10mm


Featured Industry Food and Beverages
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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