HPLC Analysis of Hormone on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Hormone on Chromolith® High Resolution RP-18e

Conditions

column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] acetonitrile; [B] 90% phosphatbuffer 10mM pH=7.0 + 10% acetonitrile
gradient Initial composition: 0-0.5min 45%A 0.5mL/min; 0.5-0.6min 45%A to 35%A 0.5mL/min; 0.6-3min 35%A 0.5mL/min to 1.5mL/min; 3.0-3.1min 35%A to 55%A 1.5mL/min; 3.1-8min 55%A 1.5mL/min
sample Prednisolone 0,6mg/ml, beta-Estradiol 0,4mg/ml, 17-alpha-Ethinyl-estradiol 0,4mg/ml, Estrone 0,5mg/ml, Progesterone 0,8mg/ml solved in Acetonitrile
injection 1 μL
detector 220 nm (cell: 11 μL)

Description

Featured Industry Life Science and Biopharma
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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