HPLC Analysis of Huaijiao Wan on Purospher(R) Star Phenyl (5mum) 150 x 4.6 mm @ 260 nm

HPLC Analysis of Huaijiao Wan on Purospher® Star Phenyl (5mum) 150 x 4.6 mm @ 260 nm

Conditions

column Purospher® Star Phenyl (5μm) 150 x 4.6 mm (1.51919.0001)
column temp. 50 °C
mobile phase [A] 2% glacial acetic acid solution; [B] mix methanol and acetonitrile 2:1 (v/v);
gradient Time (min): 0, 23, 50, 51, 57; A (%): 89, 89, 70, 10, 10; B (%): 11, 11, 30, 90, 90.
flow rate 1.0 mL/min
pressure 1131 psi (78 bar)
sample Dissolve appropriate amount of Sophoricoside, Naringin & Baicalin in methanol solution to obtain a 40, 20, 48 μg/mL (ppm) solution.
injection 10 μL
detector 260 nm cell: 1 μL/10 mm

Description

Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
Related Links