HPLC Analysis of Huaijiao Wan Tablets at 260 nm and 280 nm on Chromolith(R) HighResolution RP-18 endcapped

HPLC Analysis of Huaijiao Wan Tablets at 260 nm and 280 nm on Chromolith® HighResolution RP-18 endcapped

Conditions

sample preparation Take appropriate amount of drug pills, cut into pieces, weigh exactly 0.25g small pills to a mortar, grind four times with total 40 mL 50% methanol aqueous solution. Transfer it to a 50-mL volumetric flask, sonicate (250w, 33kHZ) for 30 minutes, cool down, weigh, make up weight with 50% methanol solution. Shake well and filter.
column Purospher® Star RP-18 endcapped, 150 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 0.1% H3PO4 aqueous solution; [B] acetonitrile; (85:15, A:B)
flow rate 1.0 mL/min
pressure 826 psi (57 bar)
sample Dilute ethanol solution (dilute 529 mL ethanol with water to 1000 mL). 60 μg/mL (60 ppm) Paeoniflorin in diluent.
injection 10 μL
detector 260-280 nm cell: 1 μL/10 mm

Description

Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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