HPLC Analysis of Hydroxymethylfurfural in Honey on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Hydroxymethylfurfural in Honey on Chromolith® High Resolution RP-18e

Conditions

sample preparation 2.5 g honey diluted in 10 mL phosphoric acid (1M)
column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] water ; (10:90, A:B)
flow rate 1.0 mL/min
pressure 900 psi (62 bar)
sample Hydroxymethylfurfural (Standard) 12,3μg/ml; Honig
injection 5 μL
detector 277 nm cell: 11 μL

Description

Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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