HPLC Analysis of Ibuprofen & Codeine Phosphate Tablet on Chromolith® High Resolution RP18e

HPLC Analysis of Ibuprofen & Codeine Phosphate Tablet on Chromolith® High Resolution RP18e

Conditions

sample preparation Weigh 20 tablet & crush. Tranfer equivalent to one tablet weight to a 100 ml volumetric flask. disperse in 70 ml methanol & sonicate for 20 min. Dilute upto the mark with methanol. Pipette out 5 ml of the above solution & dilute to 50 ml with mobile phase.
column Chromolith High Resolution RP18 endcapped, 100 x 4.6 m (1.52022.0001)
mobile phase [A] 2.24 g of KH2PO4 & 5.4 g of Sodium Octane sulfonate in 1000 ml water containing 7 ml triethyl amine adjust pH to 7.2 with orthophosphoric acid; [B] methanol; (70:30, A:B)
flow rate 1.0 mL/min
column temp. 40 °C
detector 214 nm cell: 10 μL
injection 10 μL

Description

Categories Analytical/Chromatography, Applications, HPLC Applications, Pharmaceuticals
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Materials

     
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