HPLC Analysis of Insulin on SRT® SEC-150

HPLC Analysis of Insulin on SRT® SEC-150


column SRT SEC-150, 30 cm x 7.8 mm I.D., 5 µm particles, 150 Ã… (Z777018)
column temp. Ambient
mobile phase 0.1% Arginine: ACN: Acetic Acid = 65:20:15 (V/V/V)
flow rate 0.5 mL/min
sample 4.01 mg/mLInsulin
injection 100 µL
detector UV, 214 nm


Analysis Note The analysis of insulin by gel filtration chromatography is shown on a SRT SEC-150 column. The composition of the mobile phase, 20% acetonitrile in the presence of acetic acid and arginine, indicates that insulin is a very hydrophobic peptide. At higher sensitivity the chromatogram shows several degradation products or contaminants eluting before the insulin peak, a position where one would expect compounds of higher molecular mass to elute.
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC


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