HPLC Analysis of Insulin on Chromolith Performance 100-3mm

HPLC Analysis of Insulin on Chromolith Performance 100-3mm

Conditions

sample preparation 9mg Insulin are dissolved in 9ml 0,01M HCl and put into the vaccuumdryer at 50°C for 4 hours. Then it was stored 96h by 25°C
column Chromolith Performance, 100 x 3 mm (1.52001.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] Water + 0,1% TFA v/v; (30:70, A:B)
flow rate 212 μL/min
pressure 2300 psi 159 Bar
sample Insulin 1mg/mL in 10mM H
injection 0.9 μL
detector 215 nm cell: 1.4 μL

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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