HPLC Analysis of Insulin on Chromolith Performance 100-2mm

HPLC Analysis of Insulin on Chromolith Performance 100-2mm

Conditions

sample preparation 9mg Insulin are dissolved in 9ml 0,01M HCl and put into the vaccuumdryer at 50°C for 4 hours. Then it was stored 96h by 25°C.
column Chromolith® Performance 100 x 2 mm (1.52006.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] Water + 0,1% TFA v/v; (30:70, A:B)
flow rate 95 μL/min
pressure 1581 psi (109 bar)
sample Insulin 1mg/mL in 10mM HCl
injection 0.4 μL
detector 215 nm cell: 1.4 μL

Description

Featured Industry Life Science and Biopharma
suitability application for HPLC

Materials

     
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