HPLC Analysis of Itraconazol on Chromolith(R) Performance 100-3mm

HPLC Analysis of Itraconazol on Chromolith® Performance 100-3mm

Conditions

sample preparation 50mg Itraconazol dissolved in 50mL Solution (5mL HCl 30% in 1L Methanol) to get the impurities.
column Chromolith® Performance 100 x 3 mm (1.52001.0001)
column temp. 30 °C
mobile phase [A] acetonitrile; [B] methanol; [C] 1L Water + 3,4g TBAH-sulfat; (45:10:45, A:B:C)
flow rate 850 μL/min
pressure 8193 psi (57 bar)
sample Itraconazol 1mg/1mL solution
injection 1.9 μL
detector 225 nm cell: 1.4 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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