HPLC Analysis of Itraconazol on Chromolith(R) Performance 100-2mm

HPLC Analysis of Itraconazol on Chromolith® Performance 100-2mm

Conditions

sample preparation 50mg Itraconazol dissolved in 50mL Solution (5mL HCl 30% in 1L Methanol) to get the impurities.
column Chromolith® Performance 100 x 2 mm (1.52006.0001)
column temp. 30 °C
mobile phase [A] acetonitrile; [B] methanol; [C] 1L water + 3,4g TBAH-sulfat; (45:10:45, A:B:C)
flow rate 380 μL/min
pressure 4887 psi (34 bar)
sample Itraconazol 1mg/1mL solution
injection 1.9 μL
detector 225 nm cell: 1.4 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
Related Links