HPLC Analysis of Itraconazole and Impurties on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Itraconazole and Impurties on Chromolith® High Resolution RP-18e


sample preparation 50 mg itraconazol dissolved in 50 mL solution (5 mL 30% hydrochloric acid in 1000 mL of methanol)
column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 m (1.52022.0001)
column temp. 30 °C
mobile phase [A] acetonitrile; [B] methanol; [C] L Water + 3,4g TBAH-sulfat; (45:10:45, A:B:C)
flow rate 2 mL/min
sample itraconazol (1 mg/mL)
injection 10 μL
detector 225 nm cell: 11μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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