HPLC Analysis of Lapatinib on Chromolith(R) Performance 50-2mm

HPLC Analysis of Lapatinib on Chromolith® Performance 50-2mm

Conditions

sample preparation Methanol & Acetonitrile 50:50
column Chromolith® Performance, 50 x 2 mm (1.52007.0001)
column temp. ambient
mobile phase [A] 3.48 gm of K2HPO4 in 1 lit water. pH adjusted to 7.5 with OPA; [B] Acetonitrile & Methanol 80:20;
gradient 0.0 min A --> 60%, 1.25 min A --> 60%, 4.25 min A --> 30%, 7.5 min A --> 30%, 8.25 min A --> 60%, 10.0 min A --> 60%
flow rate 0.5 mL/min
pressure 682 psi 47 Bar
sample 500 ppm of Lapatinib
injection 5.0 μL
detector 260 nm cell: 8.0 μL

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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