HPLC Analysis of Lidocaine and Related Compound A and H on Purospher(R)STAR RP-18 endcapped

HPLC Analysis of Lidocaine and Related Compound A and H on Purospher®STAR RP-18 endcapped


column Purostar RP18E 2MYM HI-HR100-2.1 mm (1.50648.0001)
column temp. 30 °C
mobile phase [A] 95/5 water / acetonitrile in 0.1% phosphoric acid; [B] 5/95 water/acetonitrile in 0.1% phosphoric acid
gradient 0% B for 2 min, then 0% to 100% B in 10 min; 100% to 0% B in 0.1 min; hold at 0% B for 4.9 min.
flow rate 0.3 mL/min
pressure 4656 psi (321 bar)
sample 100 μg/mL of lidocaine, related compound A and H in acetonitrile
injection 1 μL
detector 210 nm UV


Analysis Note Lidocaine is a medication used to numb tissue in a specific area and does not affect other areas. Topically it can be used for reducing pain by irritations such as sunburn, poison ivy, insect bites, minor cuts or even hemorrhoids. It can also be used for local anesthetic with sedative, analgesic and cardiac depressant (antiarrhythmic drug) properties This application demonstrates reverse phase interactions in a simple gradient separating lidocaine and two of its related compounds using Cerilliant CRM’s for the related compounds.
Featured Industry Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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