HPLC Analysis of Liuwei Dihuang Wan Tablets on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Liuwei Dihuang Wan Tablets on Purospher® STAR RP-18 endcapped


sample preparation Take appropriate amount of drug pills, grind, weigh 1.0g small pills to a Erlenmeyer flask with cover, add into 25 mL 50% methanol aqueous solution, weigh the total weight, heat to reflux for 30minutes, cool down, weigh, make up weight with 50% methanol aqueous solution. Shake well and filter.
column Purospher® STAR RP-18 endcapped 250 x 4.6 mm (1.51456.0001)
column temp. 40 °C
mobile phase [A] acetonitrile; [B] 0.3% H3PO4 aqueous solution;
gradient Time (min): 0,5,20,35,45,55; Solution A (%): 5,8,8,20,60,60; Solution B (%): 95,92,92,80,40.40.
flow rate 1.0 mL/min
sample Dissolve appropriate amount of each analyte to give 20 μg/mL (20 ppm) of Morroniside and Loganin and 60 μg/mL (60 ppm) Paeonol in 50% methanol solution.
injection 10 μL
detector 240 nm cell: 1μL/10 mm


Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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