HPLC Analysis of Loratadine Tablets on Chromolith(R) HighResolution RP-18 endcapped

HPLC Analysis of Loratadine Tablets on Chromolith® HighResolution RP-18 endcapped


sample preparation Take 20 loratadine tablets, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 10mg loratadine ) to a 50-mL volumetric flask, add appropriate amount of mobile phase into,shake. add H2O to volume. Filtrate.
column Chromolith® High Resolution RP-18 endcapped 100 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 2.28 g K2HPO4 in 800mL H2O, adjust pH 6.0 with H3PO4, add H2O to 1000mL; [B] methanol; (20:80, A:B)
flow rate 1 mL/min
pressure 899 psi (62 Bar)
sample Weigh appropriate amount of Loratadine to obtain 200 μg/mL (200 ppm). Dissolve with diluent.
injection 10 μL
detector 247 nm cell: 1 μL/ 10 mm


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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