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HPLC Analysis of Low MW Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size

HPLC Analysis of Low MW Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size

Conditions

column Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 µm particles (Z777016)
column Zenix SEC-100, 30 cm x 4.6 mm I.D., 3 µm particles (Z777006)
column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 °C
mobile phase 0.2 M sodium phosphate dibasic, pH 7.0 with phosphoric acid
flow rate 0.25 mL/min
pressure 1000 psi (69 Bar)
sample Agilent SEC 130Ã… std
injection 0.5 µL
detector UV, 215 nm

Description

Analysis Note Pore size is a kay parameter of resolution size exclusion chromatography. Matching the pore size to resolution of the sample components is critical in selecting a column. This application shows the impact of pore size on resolution of sample components as a function of molecular size.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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