HPLC Analysis of Lutein on Ascentis® Express C18

HPLC Analysis of Lutein on Ascentis® Express C18


sample preparation SPE (Solid Phase Extraction)
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 mum particles (53822-U)
column temp. 30 °C
mobile phase [A] Acetonitrile; [B] Water; (90:10, A:B)
flow rate 1.0 mL/min
pressure 2581 psi (178 bar)
sample 0.10 mg/mL of Lutein in 50/50 MeOH/THF
injection 0.3 µL
detector UV, 450 nm


Analysis Note The analysis of lutein is relevant to pharmaceutical, nutraceutical, food, pet foods, and animal and fish feed applications. This method uses a 2.7 μm Ascentis® Express C18 column for a rapid analysis of lutein in under two minutes with UV detection.
Categories Analytical Chromatography, Pigments
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Food and Beverages
Pharmaceutical (small molecule)
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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