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HPLC Analysis of Maté Leaves on Ascentis® Express C18

HPLC Analysis of Maté Leaves on Ascentis® Express C18


sample/matrix UAE (Ultrasonic Extraction) maté extract (dried and minced leaves (7.5 g) from maté were extracted with 225 mL of methanol in an ultrasonic bath, thermostated at 75 +/- 0.5 °C (potency: 90 W; frequency: 40 kHz))
column Ascentis Express C18, 150 x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 25 °C
mobile phase [A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid
gradient 0 to 5% B in 20 min; 5 to 40% B in 30 min
flow rate 1 mL/min
injection 10 µL
detector PDA: range 215- 420 nm (8 µL detector cell volume, cell temp. 40 °C, sampling rate 12.5 Hz, time constant 0.025 s)


Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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