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HPLC Analysis of Melamine in Infant Forumula on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Melamine in Infant Forumula on Chromolith® High Resolution RP-18e


column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 40 °C
mobile phase [A] acetonitrile; [B] pH=3 (10mM citric acid, 10mM sodium octan sulfonate; (15:85, A:B)
flow rate 0.8 mL/min
sample 10 mL infant formula spiked with 25 μg melamine
injection 2 μL
detector 240 nm cell: 11 μL


Analysis Note The injected sample is a 25 μg melamine spiked to 10 mL milk ans diluted with 1 mL acetic acid 3% v/v and stored at 5 °C for 30 min. After 15 min of centrifugation (10,000 rpm) supernatants were filtered through a 0.45 μm Millex PVDF filter and injected.
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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