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HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP

HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP


column Astec Cellulose DMP; 25 cm x 4.6 mm I.D., 5 µm particles (51099AST)
column temp. 30 °C
mobile phase [A] heptane; [B] isopropanol; (80:20, A:B)
flow rate 1 mL/min
sample mephobarbital, 1 g/L in 50:50, heptane:isopropanol
injection 15 µL
detector UV, 254 nm


Analysis Note Mephobarbital enantiomers are resolved by normal phase chromatography.
Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure
Featured Industry Pharmaceutical (small molecule)
Legal Information Astec is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Mephobarbital (aka methyphenobarbital) is used as a sedative, anxiolytic and anticonvulsant. It is the N-methylated analogue of phenobarbital, and has similiar indications, therapeutic value, and tolerability.
suitability application for HPLC


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