HPLC Analysis of Metformin & Pioglitazone on Chromolith® High Resolution RP18e

HPLC Analysis of Metformin & Pioglitazone on Chromolith® High Resolution RP18e

Conditions

column Chromolith® High Resolution RP18 endcapped, 100 x 4.6 mm (1.52022.0001)
mobile phase [A] 1.38 gm NaH2PO4 in 1800 ml water. Add 3.484 gm of Sodium Pentane Sulfonate. pH adjusted to 3.0 with OPA & dil to 2000 ml; [B] acetonitrile;
gradient 0 min % A > 90, 2.7 min % A > 90, 4.0 min % A > 60, 6.7 min % A > 60, 7.5 min % A > 90, 10.0 min % A > 90
flow rate 1.2 mL/min
column temp. 30 °C
detector 230 nm cell: 10 μL
injection 3 μL

Description

Categories Analytical/Chromatography, Applications, HPLC Applications, Pharmaceuticals
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Materials

     
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