HPLC Analysis of Methylimidazoles in Diet Cola on Ascentis® Express HILIC

HPLC Analysis of Methylimidazoles in Diet Cola on Ascentis® Express HILIC


column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 50 °C
mobile phase 2 mM ammonium formate in 5:95, water:acetonitrile, to pH 4.4 with formic acid
flow rate 0.6 mL/min
sample 200 mg/L in methanol
injection 1 µL
detector ESI(+), 50-800 m/z


Analysis Note The purpose of this study was to develop a fast analytical method for analyzing for 2-methylimidazole and 4-methylimidazole in caramel colored carbonated beverages. The FDA has recently increased the awareness of the potential hazards of methylimidazole. These compounds can be found in caramel additives used in a broad range of food stuff. In this study the focus is the levels of methylimidazole in various colas. Methylimidazoles are a difficult group of compounds to analyze due their polar nature and low molecular weight. Traditional reversed phase separation are unsuccessful in retaining these small polar compounds, with most methods utilizing ion-exchange resins for analysis. Other common methods are GC analysis with sample derivitization.
Categories Analytical Chromatography, Heterocyclic compounds
Featured Industry Food and Beverages
General description This method features fast analysis of methylimidazole derivatives used in caramel-colored carbonated beverages.
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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