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HPLC Analysis of N-Linked Glycans on BIOshell™ Glycan

HPLC Analysis of N-Linked Glycans on BIOshell™ Glycan


column BIOshell™ Glycan HPLC Column (50993-U)
column temp. 50 °C
mobile phase (A) Water with 100 mM ammonium formate, pH 4.5; (B) Acetonitrile
gradient min, %B, Flow (mL/min): 0-74-0.4; 15-71-0.4; 25-55-0.4; 26-45-0.2; 30-45-0.2; 31-75-0.4; 35-75-0.4.
pressure 1130 psi (78 bar)
sample in 70% mobile phase B
injection 3 µL
detector Fluorescence (λex = 260 nm, λem = 430 nm)


Analysis Note Separation pf a mix of 2-AB labled N-linked glycans released from human IgG was optimized on BIOshell Glycan HPLC Columns. Baseline resolutions for all of the individual glycans were observed in both mixes, except for the positional isomer pair G1F and G1F’ which were partially resolved. 
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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