HPLC Analysis of Nateglinide Tablet on Purospher STAR RP-18 endcapped

HPLC Analysis of Nateglinide Tablet on Purospher STAR RP-18 endcapped

Conditions

sample preparation Take 10 tablets of  Nagelienai Pian, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 70mg Nateglinide ) to a 100-mL volumetric flask, (add appropriate amount of 55% ACN solution. Shake for 30 minutes. Add diluent to volume. Filtrate.)
column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
column temp. 30 °C
mobile phase [A] 0.05% trifluoroacetic acid solution; [B] acetonitrile; (40:60, A:B)
flow rate 1 mL/min
pressure 1160 psi (80 bar)
sample 30 μg/mL (30 ppm) of Nateglinide in diluent to obtain the standard solution.
injection 10 μL
detector 210 nm (cell: 1 μL/10 mm)

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
Related Links