HPLC Analysis of Olmesartan Medoxomil, Amlodipine Besylate, and Hydrochlorothiazide Tablet on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Olmesartan Medoxomil, Amlodipine Besylate, and Hydrochlorothiazide Tablet on Chromolith® High Resolution RP-18e

Conditions

column Chromolith® High Resolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)
column temp. 40 °C
mobile phase [A] 2.72g of potassium dihydrogen phosphate in 1000 mL milliQ water - Add 1.0mL triethylamine. Adjust pH at 3.0 with ortho-phosphoric acid ; [B] acetonitrile; (70:30, A:B)
flow rate 1 mL/min
sample olmesartan medoxomil (0.4 mg/mL), amlodipine besylate (0.1 mg/mL), hydrochlorothiazide (0.25 mg/mL) (Resolution solution: Weigh 5.0 mg of Amlodipine Besylate in 5.0 mL volumetric flask. Add 5.0 ml hydrogen peroxide. Heat it at 70 °C for 45 mins in waterbath.)
injection 2 μL
detector 225 nm cell: 10 μL

Description

Analysis Note To prepare standards: Dilute 40 mg olmesartan medoxomil, 10 mg amlodipine besylate, and 25 mg hydrochlorothiazide to 100 mL with mobile phase component A (buffer):acetonitrile (70:30).
To prepare tablets: Crush 20 tablets. Weigh the quantity equivalent to 40 mg olmesartan, 10 mg amlodipine and 25 mg hydrochlorothiazide and transfer into a 100 ml volumetric flask. Add water and sonicate for 20 minutes. Dilute up to the mark with water. Filter through 0.45 μm filter.
Resolution solution: Weigh 5.0 mg of amlodipine besylate in 5.0 mL volumetric flask. Add 5.0 ml hydrogen peroxide. Heat at 70 °C for 45 mins in waterbath.
Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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