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HPLC Analysis of Organic Acids on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Organic Acids on Chromolith® High Resolution RP-18e

Conditions

column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 m (1.52022.0001)
column temp. 30 °C
mobile phase 0.01M Diammonium hydrogenphosphate solution( pH2.7)
flow rate 1.0 mL/min
pressure 522 psi (36 bar)
sample Prepared a mixed standards that contained 1mg/ml of Tartaric Acid,Malic Acid and Citric Acid,0.2mg/ml of Succinic acid,diluted by water (Diluent: water)
injection 20 μL
detector 210 nm cell: 1μL/10mm

Description

Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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