HPLC Analysis of Organic Acids on Chromolith(R) Performance 100-3mm

HPLC Analysis of Organic Acids on Chromolith® Performance 100-3mm


column Chromolith® Performance, 100 x 3 mm (1.52001.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] na-Phosphatbuffer 20mM pH=2,5;
gradient Initial composition: 0-0.5min 100%B to 82%B, hold until 5min, 5-6min 82%B to 100%B, hold until 8.5min, 8.5-12min 100%B to 55%B, hold until 16min
flow rate 425 μL/min
pressure 940 psi (57-65 bar)
sample Malic acid (0,5mg/ml), Fumaric acid (0,15mg/ml), Glutaric acid (0,7mg/ml), 3,4-Dihydroxy-cinnamic acid (0,1mg/ml), 4-Hydroxy-cinnamic acid (0,1mg/ml), Sorbic acid (0,75mg/ml), Benzoic acid (0,2mg/ml), 2-Hydroxybenzoic acid (0,5mg/ml), Cinnamic acid (0,2mg/ml)
injection 1 μL
detector 220 nm (cell: 1.4 μL)


Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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