HPLC Analysis of Organic Acids in Beverage on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Organic Acids in Beverage on Chromolith® High Resolution RP-18e

Conditions

column Chromolith® High Resolution RP-18 encapped, 100 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase 0.01M Diammonium hydrogenphosphate solution( pH2.7 );
flow rate 1.0 mL/min
pressure 522 psi (36 bar)
sample 5mL of Mirinda was sonicated for 5min, 0.2ml of Phosphate acid solution(1M) was added.Then the solution was diluted to 10mL by water . (Diluent: water)
injection 20 μL
detector 210 nm cell: 1 μL/10 mm

Description

Analysis Note Sample: Sonicate 5 mL of Mirinda brand soft drink for 5 min. Add 0.2 mL of phosphoric acid (1M) and dilute to 10 mL with water.
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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