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HPLC Analysis of PA-Glycans from Mouse Monoclonal Antibody (MAb) on TSKgel® Amide-80, HILIC Mode

HPLC Analysis of PA-Glycans from Mouse Monoclonal Antibody (MAb) on TSKgel® Amide-80, HILIC Mode

Conditions

column TSKgel Amide-80, 25 cm x 4.6 mm I.D., 5 µm particles (813071)
column temp. 40 °C
mobile phase [A] 0.2 M acetic acid + TEA (pH 7.3); [B] acetonitrile
gradient 75% B for 5 min; to 50% B in 75 min
flow rate 0.5 mL/min
sample pyridylaminated oligosaccharides released from mouse mAb-1
injection 50 µL
detector fluorescence, 315/380, ex/em

Description

Categories Analytical Chromatography, Carbohydrates
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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