HPLC Analysis of Peptide Mixture on Zenix® SEC-100

HPLC Analysis of Peptide Mixture on Zenix® SEC-100


column Zenix-100, 30 cm x 7.8 mm I.D., 3 µm particles, 100 Ã… (Z777008)
column temp. Ambient
mobile phase ACN:H2O:TFA=65:35:0.1(v/v)
flow rate 1.0 mL/min
sample ribonuclease A (5.931 min), human insulin (6.936 min), thymosin a1 (7.252 min), somatostatin (8.089 min)
injection 10 µL
detector UV, 214 nm


Analysis Note Zenix® SEC columns can be operated in aqueous-organic mobile phases, as is shown ion this example where hydrophobic peptides and proteins are eluted with excellent peak shape in 65% acetonitrile 35% water containing 0.1% TFA.
Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC


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