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HPLC Analysis of Peptides on Ascentis® RP-Amide

HPLC Analysis of Peptides on Ascentis® RP-Amide


column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 30 °C
mobile phase [A] 0.1% trifluoroacetic acid in water; [B] 0.1% trifluoroacetic acid in acetonitrile
gradient 9 to 21% B in 5 min; to 27% B in 15 min
flow rate 1.0 mL/min
sample as indicated in mobile phase A
injection 5 µL
detector UV, 220 nm


Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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