HPLC Analysis of Phenytoin on Chromolith(R) Performance 250 - 4.6 mm

HPLC Analysis of Phenytoin on Chromolith® Performance 250 - 4.6 mm


sample preparation Mobile phase
column Chromolith® Performance 250 x 4.6 mm (1.02129.0001)
column temp. ambient
mobile phase [A] metanol; [B] water; (50:50, A:B)
flow rate 0.5 mL/min
pressure 406 psi 28 Bar
sample 1 ppm of each Phenytoin & all related impurities
injection 10 μL
detector 229 nm cell: 10 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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