HPLC Analysis of Polyphenoles in Coffee on Chromolith(R) Performance 2 x 100-3mm

HPLC Analysis of Polyphenoles in Coffee on Chromolith® Performance 2 x 100-3mm


sample preparation 6g of coffee powder with 200ml of boiling water in a coffe-extractor.The extract was injected directly into the HPLC-System.
column Chromolith Performance 100 x 3 mm
mPurospher STAR RP-18 endcapped 150 x 4.6 mm
1 (
column temp. ambient
mobile phase [A] acetonitrile; [B] water + 0,1% Acetic acid v/v;
gradient Initial composition:0-3min 10%A, 3-14min 10%A to 50%A, hold 50%A until 20min
flow rate 850 μL/min
pressure 11213 psi 670-780 Bar
sample 6g Coffee powder in 200ml Water
injection 1.0 μL
detector 327 nm (cell: 1.4 μL)


Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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