HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)


column Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 µm particles (59601-U)
mobile phase [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate 0.5 mL/min
injection 10 mug trypsin inhibitor, 5 mug conalbumin, myoglobin or 10 mug ribonuclease A, 5 mug cytochrome c, a-chymotrypsinogen A
detector UV, 280 nm


Analysis Note The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
description Separated on Discovery® BIO PolyMA-SCX
suitability application for HPLC


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