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HPLC Analysis of Sildenafil Citrate and Related Substances on Chromolith High Resolution RP-18e

HPLC Analysis of Sildenafil Citrate and Related Substances on Chromolith High Resolution RP-18e

Conditions

column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 30 °C
mobile phase [A] 7 mL of triethylamine with water to 1mL. Adjust with phosphoric acid to a pH=3.0±0.1; [B] methanol; [C] acetonitrile; (58:25:17, A:B:C)
flow rate 1.0 mL/min
pressure 971.5 psi (67 bar)
sample Sildenafil Citrate RS 28ppm, Sildenafil impurity A 28ppm .
injection 20 μL
detector 290 nm (cell: 13 μL)

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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