HPLC Analysis of Sildenafil Citrate on Chromolith HighResolution RP-18 endcapped 150 x 4.6mm

HPLC Analysis of Sildenafil Citrate on Chromolith HighResolution RP-18 endcapped 150 x 4.6mm

Conditions

sample preparation Dissolve 70 mg of Sildenafil Citrate in 1 mL of a solution of hydrogen peroxide and anhydrous formic acid (2:1). (Allow to stand for at least 10 min to generate sildenafil N-oxide, and then dilute with Mobile phase to 250 mL. )
column Chromolith HighResolution RP-18 endcapped, 150 x 4.6 mm (1.52023.0001)
column temp. 30 °C
mobile phase [A] Dilute 7 mL of triethylamine with water to 1 L. Stir, and adjust with phosphoric acid to a pH of 3.0 ± 0.1; [B] methanol; [C] acetonitrile; (58:25:17, A:B:C)
flow rate 1.5 mL/min
pressure 1160 psi (80 bar)
sample 0.7 mg/mL of Sildenafil Citrate in Mobile phase
injection 20 μL
detector 290 nm cell: 10 μL

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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