HPLC Analysis of Simvastatine USP on Chromolith High Resolution RP-18e (analyte set 1)

HPLC Analysis of Simvastatine USP on Chromolith High Resolution RP-18e (analyte set 1)

Conditions

sample preparation 1.4mg/ml monobasic potassium phosphate in water. Diluent: Acetonitril and Buffer 3:2
column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase [A] 50% acetonitril, 50% posphoric acid and water 1:999; [B] phosphoric acid and Acetonitril 1:999
gradient 100% A held for 4.5 min; to 95% A for 3.5 min; to 25% A for 3.5 min; to 100% A for 1.5 mins; (Original: Initial composition:0-4.5min 100%A, 4.6min to 8 min 95%A to 25%A, 8min to 11.5min 25%A, 11.6min to 13min 100%A)
flow rate 3 mL/min
sample Lovastatin (1,5mg/ml), Simvastatin (1,5mg/ml)
injection 5 μL
detector 238 nm cell: 11 μL

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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