HPLC Analysis of Simvastatine USP on Chromolith(R) Performance SpeedRod 50-4.6mm

HPLC Analysis of Simvastatine USP on Chromolith® Performance SpeedRod 50-4.6mm


sample preparation 1.4mg/ml monobasic potassium phosphate in water
column Chromolith® Performance SpeedRod 50-4.6mm (1.51450.0001)
column temp. ambient
mobile phase [A] 50% Acetonitril, 50% phosphoric acid and water 1:999; [B] 100% phosphoric acid and Acetonitril 1:999;
gradient Initial composition:0-4.5min 100%A, 4.6min to 8 min 95%A to 25%A (8min to 11.5min 25%A, 11.6min to 13min 100%A)
flow rate 3 mL/min
pressure 5462 psi (20-38 bar)
sample Lovastatin (1,5mg/ml), Simvastatin (1,5mg/ml) (Diluent: Acetonitril and Buffer 3:2)
injection 5 μL
detector 238 nm cell: 11 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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