HPLC Analysis of Spice Cannabinoids on Ascentis® Express F5

HPLC Analysis of Spice Cannabinoids on Ascentis® Express F5


column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 30 °C
mobile phase [A] 50 mM ammonium formate; [B] water; [C] acetonitrile; (10:35:55, A:B:C)
flow rate 0.6 mL/min
pressure 4075 psi (281 bar)
sample 100 µg/mL in 45:55 water:acetonitrile
injection 3 µL
detector UV, 200 nm


Analysis Note Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provide a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of eight of these compounds on Ascentis Express F5 column is shown here. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, drugs of abuse, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


Related Links