HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18

HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 30 °C
mobile phase [A] 5 mM ammonium formate water; [B] 5 mM ammonium formate (95:5, acetonitrile:water)
gradient 68 to 100% B in 8 min; held at 100% B for 2 min
flow rate 0.6 mL/min
pressure 4351 psi (300 bar)
injection 3 µL
detector UV, 265 nm


Analysis Note Synthetic cannabinoids (e.g. "Spice") are a type of designer drug used to get a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds in the blood or urine suspected users is a significant analytical challenge facing forensic chemists. Here is shown the selectivity provided by C18 phase chemistry on an Ascentis Express C18 column with UV detection. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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