HPLC Analysis of Spironolactone on Chromolith(R) HighResolution RP-18 endcapped 150 x 4.6mm

HPLC Analysis of Spironolactone on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

Conditions

column Chromolith® High Resolution RP-18 encapped, 150 x 4.6 mm (1.52023.0001)
column temp. ambient
mobile phase [A] water; [B] methanol; (40:60, A:B)
flow rate 1.0 mL/min
pressure 972 psi (67 bar)
sample 0.5 mg/mL (500 ppm) of Spironolactone RS in diluent.
0.5 mg/mL  (500 ppm) of Spironolactone RS in diluent.
Diluent: Acetonitrile/Water 1:1
( (Diluent: Acetonitrile/Water 1:1 (v/v))
injection 20 μL
detector 230 nm cell: 11μL

cell: 13 μL

Description

Featured Industry Life Science and Biopharma
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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