HPLC Analysis of Spironolactone on Chromolith(R) HighResolution RP-18 endcapped 150 x 4.6 mm

HPLC Analysis of Spironolactone on Chromolith® HighResolution RP-18 endcapped 150 x 4.6 mm

Conditions

column Chromolith® HighResolution RP-18 endcapped, 150 x 4.6 mm (1.52023.0001)
column temp. ambient
mobile phase [A] water; [B] methanol; (40:60, A:B)
flow rate 1.5 mL/min
pressure 1595 psi (110 bar)
sample 000.5 mg/mL (500 ppm) of Spironolactone RS in diluent.
0.5 mg/mL (500 ppm) of Spironolactone RS in diluent. diluent: acetonitrile/water 1:1
( (diluent: acetonitrile/water 1:1 (v/v))
injection 20 μL
detector 230 nm cell: 11 μL

Description

Featured Industry Life Science and Biopharma
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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