HPLC Analysis of Spironolactone on Chromolith(R) HighResolution RP-18 endcapped 100 x 4.6 mm

HPLC Analysis of Spironolactone on Chromolith® HighResolution RP-18 endcapped 100 x 4.6 mm

Conditions

column Chromolith® HighResolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase [A] water; [B] methanol; (40:60, A:B)
flow rate 1.5 mL/min
pressure 1175 psi (81 bar)
sample 0.5 mg/mL (500 ppm) of Spironolactone RS in diluent (diluent: acetonitrile/water 1:1 (v/v))
injection 13 μL (reduced with 33% as it is scaled to a 33% shorter column with same diameter)
detector 230 nm (cell: 11 μL)

Description

Featured Industry Life Science and Biopharma
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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