HPLC Analysis of Spironolactone on Chromolith(R) HighResolution RP-18 endcapped 50 x 4.6 mm

HPLC Analysis of Spironolactone on Chromolith® HighResolution RP-18 endcapped 50 x 4.6 mm

Conditions

column Chromolith® HighResolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)
column temp. ambient
mobile phase [A] water; [B] methanol; (40:60,:A:B)
flow rate 1.5 mL/min
pressure 856 psi (59 bar)
sample 0.5 mg/mL, 500 ppm Spironolactone RS in diluent (diluent: acetonitrile/water 1:1 (v/v))
injection 7 μL (reduced with 67% as it is scaled to a 67% shorter column with same diameter)
detector 230 nm cell: 11μL

Description

Featured Industry Life Science and Biopharma
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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